The Carbohydrate-binding Specificity of a Highly Toxic Protein from Abrus pulchellus Seeds

The carbohydrate-binding specificity of a highly toxic protein from Abrus pulchellus seeds.

Carbohydrate binding specificity of the B-cell maturation mitogen from Artocarpus integrifolia seeds.

Artocarpin, a mannose-specific lectin, is a homotetrameric protein (M(r) 65,000) devoid of covalently attached carbohydrates and consists of four isolectins with pI in the range 5-6.5. Investigations of its carbohydrate binding specificity reveal that among monosaccharides, mannose is preferred over glucose. Among mannooligosaccharides, mannotriose (Man alpha 1-3[Man alpha 1-6]Man) and mannopen...

Plant Growth Inhibitor from Abrus precatorius Seeds.

Binding specificity of mannose-specific carbohydrate-binding protein from the cell surface of Trypanosoma cruzi.

The sugar binding specificity of the recently described mannose-specific carbohydrate-binding proteins (CBP) isolated to homogeneity from both the epimastigote and trypomastigote stages of the pathogenic protozoa Trypanosoma cruzi has been studied by quantitative hapten inhibition of the biotinylated CBPs to immobilized thyroglobulin using model oligosaccharides. The results clearly show a diff...

Carbohydrate binding studies on the lectin from Datura stramonium seeds.

The carbohydrate-binding properties of the Datura stramonium seed lectin were studied by equilibrium dialysis, quantitative precipitation of natural and synthetic glycoproteins, and hapten inhibition of precipitation. The dimeric lectin (Mr = 86,000) possesses two carbohydrate-binding sites for N,N'N",N"'- tetraacetylchitotetritol /mol protein, with an apparent Ka = 8.7 X 10(3) M-1 at 4 degrees...